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 | Acesso ao texto completo restrito à biblioteca da Embrapa Gado de Leite. Para informações adicionais entre em contato com cnpgl.biblioteca@embrapa.br. |
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Registro Completo |
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Biblioteca(s): |
Embrapa Gado de Leite. |
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Data corrente: |
13/08/2021 |
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Data da última atualização: |
13/08/2021 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Autoria: |
OLIVEIRA, C. S.; FEUCHARD, V. L. da S.; FREITAS, C. de; ROSA, P. M. da S.; CAMARGO, A. J. dos R.; SARAIVA, N. Z. |
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Afiliação: |
CLARA SLADE OLIVEIRA, CNPGL; VIVIANE LUZIA DA SILVA FEUCHARD, Pesagro; CELIO DE FREITAS, CNPGL; PAOLA MARIA DA SILVA ROSA; AGOSTINHO JORGE DOS REIS CAMARGO, Pesagro; NAIARA ZOCCAL SARAIVA, CNPGL. |
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Título: |
In-straw warming protocol improves survival of vitrified embryos and allows direct transfer in cattle. |
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Ano de publicação: |
2020 |
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Fonte/Imprenta: |
Cryobiology, v. 97, p. 222-225, 2020. |
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DOI: |
https://doi.org/10.1016/j.cryobiol.2020.02.007 |
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Idioma: |
Inglês |
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Conteúdo: |
Vitrification is a superior method for cryopreservation of IVF embryos, but due to complicated warming protocols, it is not commonly used for commercial bovine embryos routine. To overcome the need of laboratory embryo preparation during warming, we developed an in-straw warming protocol compatible with most vitrification devices for embryo transfer without sucrose gradient steps and embryo evaluation. Surprisingly, one of the tested protocols improved embryo survival (95.0%* vs 83.1% expansion rate and 74.2%* vs 51.5% hatching rate) compared to conventional in-plate warming. Embryo quality was also increased, taken by the higher total cell numbers (160.7 ± 8.6* vs 99.0 ± 7.9) and lower apoptosis index (4.9 ±0.6* vs 11.5 ± 2.4) 48 h after warming. Pregnancy rates were similar between vitrified-warmed embryos and fresh embryos (40% vs 43%). Based on our results, we suggest in-straw warming should always be used for vitrified embryos due to beneficial effects. Direct transfer can be safely performed using this protocol. |
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Palavras-Chave: |
Criotolerância; Fertilização in vitro; Vitrificação. |
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Thesagro: |
Bovino; Criopreservação; Embrião Animal; Palha; Reprodução Animal; Sacarose. |
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Categoria do assunto: |
L Ciência Animal e Produtos de Origem Animal |
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Marc: |
LEADER 01940naa a2200301 a 4500
001 2133567
005 2021-08-13
008 2020 bl uuuu u00u1 u #d
024 7 $ahttps://doi.org/10.1016/j.cryobiol.2020.02.007$2DOI
100 1 $aOLIVEIRA, C. S.
245 $aIn-straw warming protocol improves survival of vitrified embryos and allows direct transfer in cattle.$h[electronic resource]
260 $c2020
520 $aVitrification is a superior method for cryopreservation of IVF embryos, but due to complicated warming protocols, it is not commonly used for commercial bovine embryos routine. To overcome the need of laboratory embryo preparation during warming, we developed an in-straw warming protocol compatible with most vitrification devices for embryo transfer without sucrose gradient steps and embryo evaluation. Surprisingly, one of the tested protocols improved embryo survival (95.0%* vs 83.1% expansion rate and 74.2%* vs 51.5% hatching rate) compared to conventional in-plate warming. Embryo quality was also increased, taken by the higher total cell numbers (160.7 ± 8.6* vs 99.0 ± 7.9) and lower apoptosis index (4.9 ±0.6* vs 11.5 ± 2.4) 48 h after warming. Pregnancy rates were similar between vitrified-warmed embryos and fresh embryos (40% vs 43%). Based on our results, we suggest in-straw warming should always be used for vitrified embryos due to beneficial effects. Direct transfer can be safely performed using this protocol.
650 $aBovino
650 $aCriopreservação
650 $aEmbrião Animal
650 $aPalha
650 $aReprodução Animal
650 $aSacarose
653 $aCriotolerância
653 $aFertilização in vitro
653 $aVitrificação
700 1 $aFEUCHARD, V. L. da S.
700 1 $aFREITAS, C. de
700 1 $aROSA, P. M. da S.
700 1 $aCAMARGO, A. J. dos R.
700 1 $aSARAIVA, N. Z.
773 $tCryobiology$gv. 97, p. 222-225, 2020.
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Registro original: |
Embrapa Gado de Leite (CNPGL) |
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Biblioteca |
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Origem |
Tipo/Formato |
Classificação |
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Voltar
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| Acesso ao texto completo restrito à biblioteca da Embrapa Florestas. Para informações adicionais entre em contato com cnpf.biblioteca@embrapa.br. |
Registro Completo
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Biblioteca(s): |
Embrapa Florestas. |
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Data corrente: |
19/02/2024 |
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Data da última atualização: |
03/05/2024 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Circulação/Nível: |
B - 1 |
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Autoria: |
AGUIAR, N. S. de; GABIRA, M. M.; DUARTE, M. M.; TOMASI, J. de C.; HANSEL, F. A.; LAVORANTI, O. J.; DESCHAMPS, C.; HELM, C. V.; WENDLING, I. |
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Afiliação: |
NATALIA SAUDADE DE AGUIAR, UNIVERSIDADE FEDERAL DO PARANÁ; MÔNICA MORENO GABIRA, UNIVERSIDADE FEDERAL DO PARANÁ; MANOELA MENDES DUARTE, BOLSISTA NA EMBRAPA FLORESTAS; JÉSSICA DE CÁSSIA TOMASI, UNIVERSIDADE FEDERAL DO PARANÁ; FABRICIO AUGUSTO HANSEL, CNPF; OSMIR JOSE LAVORANTI, CNPF; CICERO DESCHAMPS, UNIVERSIDADE FEDERAL DO PARANÁ; CRISTIANE VIEIRA HELM, CNPF; IVAR WENDLING, CNPF. |
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Título: |
How shading levels affect bioactive compounds in leaves of yerba mate clones. |
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Ano de publicação: |
2024 |
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Fonte/Imprenta: |
Biochemical Systematics and Ecology, v. 113, 104796, 2024. |
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DOI: |
https://doi.org/10.1016/j.bse.2024.104796 |
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Idioma: |
Inglês |
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Conteúdo: |
Shading is one of the factors that influences the production of bioactive compounds in yerba mate leaves (Ilex paraguariensis). In this study, we simulated different shading levels in a new yerba mate cultivation system, semi-hydroponic inside a greenhouse. We evaluated how five levels of artificial shading influence on the bioactive compounds methylxanthines and caffeoylquinic acids (CQAs) in young and mature leaves of two yerba mate clones (EC22 and EC40) grown in a semi-hydroponic system over six harvests. From the aqueous extract, we determined the contents of methylxanthines and CQAs on an Ultra-Fast Liquid Chromatograph (UFLC), and the annual productivity of compounds per productive area was calculated considering leaf mass production m−2. There was a higher influence of clone and leaf type on the levels of bioactive compounds, especially methylxanthines – young leaves and EC40 clone showed higher caffeine content. The clones responded differently to shading: higher shading levels favor caffeine accumulation in the EC40 clone; and in the EC22 clone the absence of shading increased theobromine content. Despite the different effects of shading on each compound and clone, there is a reduction in the annual productivity of all bioactive compounds with increasing shading due to the low leaf mass productivity. Thus, we do not recommend shading in the yerba mate semi-hydroponic cultivation. This cultivation system guarantees high productivity of bioactive compounds, especially CQAs. Our study demonstrates yerba mate chemical potential to generate numerous products, including industrial extraction of methylxanthines and CQAs, increasing the commercial value of the species. MenosShading is one of the factors that influences the production of bioactive compounds in yerba mate leaves (Ilex paraguariensis). In this study, we simulated different shading levels in a new yerba mate cultivation system, semi-hydroponic inside a greenhouse. We evaluated how five levels of artificial shading influence on the bioactive compounds methylxanthines and caffeoylquinic acids (CQAs) in young and mature leaves of two yerba mate clones (EC22 and EC40) grown in a semi-hydroponic system over six harvests. From the aqueous extract, we determined the contents of methylxanthines and CQAs on an Ultra-Fast Liquid Chromatograph (UFLC), and the annual productivity of compounds per productive area was calculated considering leaf mass production m−2. There was a higher influence of clone and leaf type on the levels of bioactive compounds, especially methylxanthines – young leaves and EC40 clone showed higher caffeine content. The clones responded differently to shading: higher shading levels favor caffeine accumulation in the EC40 clone; and in the EC22 clone the absence of shading increased theobromine content. Despite the different effects of shading on each compound and clone, there is a reduction in the annual productivity of all bioactive compounds with increasing shading due to the low leaf mass productivity. Thus, we do not recommend shading in the yerba mate semi-hydroponic cultivation. This cultivation system guarantees high productivity of bioactive compounds, especiall... Mostrar Tudo |
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Palavras-Chave: |
Ácidos cafeoilquínicos; Caffeoylquinic acids; Incidência de luz; Light incidence; Metabólitos secundários; Methylxanthines; Metilxantinas. |
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Thesagro: |
Clone; Ilex Paraguariensis. |
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Thesaurus NAL: |
Clones; Secondary metabolites. |
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Categoria do assunto: |
K Ciência Florestal e Produtos de Origem Vegetal |
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Marc: |
LEADER 02762naa a2200361 a 4500
001 2162089
005 2024-05-03
008 2024 bl uuuu u00u1 u #d
024 7 $ahttps://doi.org/10.1016/j.bse.2024.104796$2DOI
100 1 $aAGUIAR, N. S. de
245 $aHow shading levels affect bioactive compounds in leaves of yerba mate clones.$h[electronic resource]
260 $c2024
520 $aShading is one of the factors that influences the production of bioactive compounds in yerba mate leaves (Ilex paraguariensis). In this study, we simulated different shading levels in a new yerba mate cultivation system, semi-hydroponic inside a greenhouse. We evaluated how five levels of artificial shading influence on the bioactive compounds methylxanthines and caffeoylquinic acids (CQAs) in young and mature leaves of two yerba mate clones (EC22 and EC40) grown in a semi-hydroponic system over six harvests. From the aqueous extract, we determined the contents of methylxanthines and CQAs on an Ultra-Fast Liquid Chromatograph (UFLC), and the annual productivity of compounds per productive area was calculated considering leaf mass production m−2. There was a higher influence of clone and leaf type on the levels of bioactive compounds, especially methylxanthines – young leaves and EC40 clone showed higher caffeine content. The clones responded differently to shading: higher shading levels favor caffeine accumulation in the EC40 clone; and in the EC22 clone the absence of shading increased theobromine content. Despite the different effects of shading on each compound and clone, there is a reduction in the annual productivity of all bioactive compounds with increasing shading due to the low leaf mass productivity. Thus, we do not recommend shading in the yerba mate semi-hydroponic cultivation. This cultivation system guarantees high productivity of bioactive compounds, especially CQAs. Our study demonstrates yerba mate chemical potential to generate numerous products, including industrial extraction of methylxanthines and CQAs, increasing the commercial value of the species.
650 $aClones
650 $aSecondary metabolites
650 $aClone
650 $aIlex Paraguariensis
653 $aÁcidos cafeoilquínicos
653 $aCaffeoylquinic acids
653 $aIncidência de luz
653 $aLight incidence
653 $aMetabólitos secundários
653 $aMethylxanthines
653 $aMetilxantinas
700 1 $aGABIRA, M. M.
700 1 $aDUARTE, M. M.
700 1 $aTOMASI, J. de C.
700 1 $aHANSEL, F. A.
700 1 $aLAVORANTI, O. J.
700 1 $aDESCHAMPS, C.
700 1 $aHELM, C. V.
700 1 $aWENDLING, I.
773 $tBiochemical Systematics and Ecology$gv. 113, 104796, 2024.
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